大鼠热结肠道型气分证模型的构建及肠道结构、IEL和GC数量的变化研究

doi:10.11843/j.issn.0366-6964.2014.04.024

ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (4): 672-678.doi: 10.11843/j.issn.0366-6964.2014.04.024

Study on Construction of Heat Accumulation of Intestinal Type of Qi-fen Syndrome and Changes of Intestinal Structure and Number of IEL，GC in Rat

WANG Zi-li^1,2，HE Ting-zhen¹，ZHANG Zhu¹，WANG Chen-chao¹，WANG Ming-zhou¹，TU Jun-ping¹，Selma Shilongo¹，ZHANG Jia-hua¹，BIE Ying-tang²

(1.College of Animal Science and Technology，Southwest University，Chongqing 400715， China;2.Chongqing Everbright Group Limited Company，Chongqing 400020， China)

Received:2013-10-08 Online:2014-04-23 Published:2014-04-23

Abstract

Abstract:

In order to construct the model of heat accumulation of intestinal type of Qi-fen Syndrome，the rats were feed with the hot medicine and the rat wastes，then were injected with the inactive E.coli，and the clinic symptoms such as the in taking，drinking，body weight and body temperature were determined，even the intestinal organizational structure，and the number of intestinal IEL and GC in rat were studied.According to the clinic symptoms，in taking，drinking，body temperature of the rats，the results showed that that the methods of feeding with the hot medicine，wastes and injecting the inactive E.coli to the rats could make the model of heat accumulation of intestinal type of Qi-fen Syndrome in successfully when the rats were injected with the inactive E.coli 5 hours later.The changes of intestinal structure in rat injected with the inactive E.coli 5 hours later were significantly，and the numbers of the IEL and GC in the duodenum，jejunum and ileum decreased in significantly.Therefore，the model of heat accumulation of intestinal type of Qi-fen Syndrome in rat can be made in successfully after the rats were injected with the inactive E.coli 5 hours later.

CLC Number:

S853.1

WANG Zi-li，HE Ting-zhen，ZHANG Zhu，WANG Chen-chao，WANG Ming-zhou，TU Jun-ping，Selma Shilongo，ZHANG Jia-hua，BIE Ying-tang. Study on Construction of Heat Accumulation of Intestinal Type of Qi-fen Syndrome and Changes of Intestinal Structure and Number of IEL，GC in Rat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 672-678.

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Study on Construction of Heat Accumulation of Intestinal Type of Qi-fen Syndrome and Changes of Intestinal Structure and Number of IEL，GC in Rat

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